孙佳,毛 燕,李刚,张 丽.应用亲水作用液相色谱法测定食品中过氧化氢[J].食品安全质量检测学报,2013,4(6):1873-1879
应用亲水作用液相色谱法测定食品中过氧化氢
Application of hydrophilic interaction liquid chromatography to determine hydrogen peroxide in food
投稿时间:2013-09-15  修订日期:2013-11-04
DOI:
中文关键词:  过氧化氢  食品  液相色谱法  HILIC色谱柱
英文关键词:hydrogen peroxide  food  high pressure liquid chromatography  HILIC column
基金项目:吉林省质量技术监督科技项目(2012-ZJK10)
作者单位
孙佳 吉林农业大学食品与科学工程学院 
毛 燕 吉林省产品质量监督检验院 
李刚 1. 吉林农业大学食品与科学工程学院; 2. 吉林省产品质量监督检验院 
张 丽 吉林农业大学食品与科学工程学院 
AuthorInstitution
SUN Jia College of Food Science and Technology, Jilin Agricultural University 
MAO Yan Jilin Province Product Quality Supervision Test Institute 
LI Gang 1. College of Food Science and Technology, Jilin Agricultural University;2. Jilin Province Product Quality Supervision Test Institute 
ZHANG Li College of Food Science and Technology, Jilin Agricultural University 
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中文摘要:
      目的 建立食品中过氧化氢含量的高压液相色谱检测方法。方法 用水提取样品中过氧化氢, 用乙腈做萃取液和流动相, 在1 mL/min流速下, 用Venusil HILIC 色谱柱(250 mm×4.6 mm, 5 μm, 100 ?)不经过衍生化, 直接通过紫外检测器检测食品中的过氧化氢。结果 过氧化氢的检出限为0.6 μg/mL, 定量限为2 μg/mL。对实际样品进行检测时, 平均回收率R≥30%, RSD≤7%。结论 该方法准确、灵敏、精密度高, 可用于食品中过氧化氢的测定。
英文摘要:
      Objective To establish a high pressure liquid chromatography (HPLC) method for determination of the hydrogen peroxide content in food. Methods The food sample was directly extracted with water, and the residual hydrogen perioxide (H2O2) was determined without derivatization via hydrophilic interaction chromatography (HILIC) subsequently. The separation was carried out through a Venusil HILIC column (250 mm×4.6 mm, 5 μm, 100 ?) with acetonitrile as mobile phase at a flow rate of 1 mL/min. Results The detection limit and quantitation limit of H2O2 were 0.6 μg/mL and 2 μg/mL, respectively. The average recovery of food sample was higher than 30% with relative standard deviation (RSD) of lower than 7%. Conclusion This method has a high sensitivity, a good precision and accuracy, which can be applied to determine the H2O2 in food.
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