王露,杭学宇,宋鑫,王芹,冯晓青,徐瑞.新型磁性分子印迹-高效液相色谱法测定牛奶中的环丙沙星[J].食品安全质量检测学报,2018,9(15):3999-4005
新型磁性分子印迹-高效液相色谱法测定牛奶中的环丙沙星
Determination of ciprofloxacin in milk by new magnetic molecular imprinting- high performance liquid chromatography
投稿时间:2018-05-07  修订日期:2018-08-07
DOI:
中文关键词:  CoFe2O4–石墨烯  磁性分子印迹  环丙沙星  高效液相色谱法
英文关键词:CoFe2O4–graphene  magnetic molecularly imprinted polymers  ciprofloxacin  high performance liquid chromatography
基金项目:江苏省卫计委预防医学科研课题(Y2015035)、2017年淮安市科技计划(指导性)项目(HABZ201732)
作者单位
王露 淮安市疾病预防控制中心 
杭学宇 淮安市疾病预防控制中心 
宋鑫 淮安市疾病预防控制中心 
王芹 淮安市疾病预防控制中心 
冯晓青 淮安市疾病预防控制中心 
徐瑞 淮安市疾病预防控制中心 
AuthorInstitution
WANG Lu Huaian Center for Disease Control and Prevention 
HANG Xue-Yu Huaian Center for Disease Control and Prevention 
SONG Xin Huaian Center for Disease Control and Prevention 
WANG Qin Huai’an Center for Disease Control and Prevention 
FENG Xiao-Qing Huai’an Center for Disease Control and Prevention 
XU Rui Huai’an Center for Disease Control and Prevention 
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中文摘要:
      目的 建立新型磁性分子印迹聚合物-高效液相色谱法测定牛奶中的环丙沙星的含量。方法 以CoFe2O4-石墨烯为载体, 多巴胺为功能单体, 环丙沙星为模板分子合成新型磁性分子印迹材料。牛奶样品经乙腈提取后, 采用该新型磁性分子印迹材料吸附其中的环丙沙星, 经甲醇-乙酸(9:1,V:V)混合溶液洗脱, 过膜后进入高效液相色谱仪进行定量测定。结果 该新型磁性分子印迹材料在15 min内能快速达到吸附平衡且识别性较好。在最佳实验条件下, 在环丙沙星浓度为0.5~20 μg/L范围内线性关系良好(r2=0.9986), 检出限为 0.09 μg/L, 平均回收率为91%~105.1%, 相对标准偏差为1.9%~4.7%。结论 该方法操作简便, 选择性好可用于牛奶中环丙沙星的定量检测。
英文摘要:
      Objective To establish a method for determination of the content of ciprofloxacin in milk by new magnetic molecular imprinting-high performance liquid chromatography. Methods The new magnetic molecular imprinting material was synthesized with CoFe2O4--graphene as the carrier, dopamine as the functional monomer and ciprofloxacin as the template molecule. After the milk sample was extracted with acetonitrile, the new magnetic molecular imprinting material was used to adsorb the ciprofloxacin. The solution was eluted by methanol-acetic acid (9:1, V:V), filtered through membrane, and quantitative determination was carried out by high performance liquid chromatograph. Results The new magnetic molecular imprinting material could reach the adsorption equilibrium rapidly within 15 minutes and had good identification. Under the best experimental conditions, ciprofloxacin showed a good rrelationship between peak areas and concentrations in the concentration range of 0.5-20 μg/L (r2=0.9986) and the limit of detection was 0.09 μg/L. The recoveries were 91%-105.1% with relative standard deviation of 1.9%-4.7%. Conclusion This method is simple to operate and can be used for quantitative detection of ciprofloxacin in milk.
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