赵唯雯,高梦楠,黄汉昌,常 平.高效液相色谱法测定保健食品中的虾青素含量[J].食品安全质量检测学报,2021,12(6):2229-2234
高效液相色谱法测定保健食品中的虾青素含量
Determination of astaxanthin in functional food by high performance liquid chromatography
投稿时间:2020-12-14  修订日期:2021-01-16
DOI:
中文关键词:  虾青素  高效液相色谱法  保健食品
英文关键词:astaxanthin  high performance liquid chromatography  functional food
基金项目:北京联合大学科研项目(JZ10202001)
作者单位
赵唯雯 北京联合大学应用文理学院保健食品功能检测中心;北京联合大学生物活性物质与功能北京市重点实验室 
高梦楠 北京联合大学应用文理学院保健食品功能检测中心;北京联合大学生物活性物质与功能北京市重点实验室 
黄汉昌 北京联合大学应用文理学院保健食品功能检测中心;北京联合大学生物活性物质与功能北京市重点实验室 
常 平 北京联合大学应用文理学院保健食品功能检测中心;北京联合大学生物活性物质与功能北京市重点实验室 
AuthorInstitution
ZHAO Wei-Wen Testing Center of Health Food Function Determination, College of Arts and science of Beijing Union University;Beijing Key laboratory of Bioactive Substances and Functional Food, Research Institute of Science and Technology of Functional Food, Beijing Union University 
GAO Meng-Nan Testing Center of Health Food Function Determination, College of Arts and science of Beijing Union University;Beijing Key laboratory of Bioactive Substances and Functional Food, Research Institute of Science and Technology of Functional Food, Beijing Union University 
HUANG Han-Chang Testing Center of Health Food Function Determination, College of Arts and science of Beijing Union University;Beijing Key laboratory of Bioactive Substances and Functional Food, Research Institute of Science and Technology of Functional Food, Beijing Union University 
CHANG Ping Testing Center of Health Food Function Determination, College of Arts and science of Beijing Union University;Beijing Key laboratory of Bioactive Substances and Functional Food, Research Institute of Science and Technology of Functional Food, Beijing Union University 
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中文摘要:
      目的 建立高效液相色谱法检测保健食品中虾青素含量的分析方法。方法 采用油状形态和粉末状形态样品来源的保健食品, 样品经二氯甲烷-甲醇混合溶液(1:3, V:V)提取、NaOH甲醇溶液皂化后, 经高效液相色谱分离、紫外可见光检测器检测。结果 全反式虾青素在0.25~12.70 μg/mL范围内呈现良好的线性关系, 检出限为1.09 ng/mL, 定量限为3.64 ng/mL。油状形态样品虾青素的精密度为2.4%、回收率为96.7%。粉末形态样品虾青素的精密度为2.0%、回收率为98.6%。在21.9 mmol/L以下浓度范围内NaOH不会造成虾青素的降解。结论 该方法回收率高、精密度良好, 测量结果准确, 可以应用于保健食品中虾青素含量的测定。
英文摘要:
      Objective To establish a method for the determination of astaxanthin in health food by high performance liquid chromatography. Methods Sample sources of health foods in oil form and powder form were used. The samples were extracted by dichloromethane methanol mixed solution (1:3, V:V) and saponified with NaOH methanol solution. The samples were separated by high performance liquid chromatography and detected by ultraviolet-visible detector. Results All trans astaxanthin showed good linear relationship in the range of 0.25?12.70 μg/mL, the limit of detection was 1.09 ng/mL, and the limit of quantification was 3.64 ng/mL. The precision and recovery of astaxanthin in oil sample were 2.4% and 96.7%, respectively. The precision and recovery of astaxanthin in powder sample were 2.0% and 98.6%, respectively. NaOH did not degrade astaxanthin in the concentration range below 21.9 mmol/L. Conclusion This methodhas high recovery rate, good precision and accurate measurement results, and is suitable for the determination of astaxanthin in healthy food.
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