江利华,刘佳惠,颜朦朦,李腾飞,朱 超,李林森,屈 锋.基于核酸适配体的胶体金比色法检测奶粉中的乳铁蛋白[J].食品安全质量检测学报,2022,13(9):2864-2870
基于核酸适配体的胶体金比色法检测奶粉中的乳铁蛋白
Determination of lactoferrin in milk powder by colloidal gold colorimetry based on nucleic acid aptamer
投稿时间:2022-01-21  修订日期:2022-04-21
DOI:
中文关键词:  乳铁蛋白  适配体  胶体金  比色法
英文关键词:lactoferrin  aptamer  AuNPs  colorimetric method
基金项目:北京市科协2020-2022年度青年人才托举工程、山东省农业科学院创新工程项目(CXGC2021B14, CXGC2021A19)、国家自然科学基金项目(3210161967)
作者单位
江利华 河北工程大学生命科学与食品工程学院, 天然产物与功能食品开发重点实验室 
刘佳惠 河北工程大学生命科学与食品工程学院, 天然产物与功能食品开发重点实验室;山东省农业科学院, 农业质量标准与检测技术研究所 
颜朦朦 山东省农业科学院, 农业质量标准与检测技术研究所 
李腾飞 河北工程大学生命科学与食品工程学院, 天然产物与功能食品开发重点实验室 
朱 超 山东省农业科学院, 农业质量标准与检测技术研究所 
李林森 北京理工大学生命学院 
屈 锋 北京理工大学生命学院 
AuthorInstitution
JIANG Li-Hua Laboratory of Natural Products and Functional Food Development, Life Sciences and Food Engineering, Hebei University of Engineering 
LIU Jia-Hui Laboratory of Natural Products and Functional Food Development, Life Sciences and Food Engineering, Hebei University of Engineering;Institute of Quality Standard and Testing Technology for Agro-products, Shandong Academy of Agricultural Sciences 
YAN Meng-Meng Institute of Quality Standard and Testing Technology for Agro-products, Shandong Academy of Agricultural Sciences 
LI Teng-Fei Laboratory of Natural Products and Functional Food Development, Life Sciences and Food Engineering, Hebei University of Engineering 
ZHU Chao Institute of Quality Standard and Testing Technology for Agro-products, Shandong Academy of Agricultural Sciences 
LI Lin-Sen School of Life Science, Beijing Institute of Technology 
QU Feng School of Life Science, Beijing Institute of Technology 
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中文摘要:
      目的 建立一种基于核酸适配体胶体金(colloidal gold, AuNPs)比色法检测奶粉中乳铁蛋白(lactoferrin, LF)的方法。方法 以无标记的LF适配体为生物识别元件, AuNPs为信号分子, 利用AuNPs的光学特性构建一种可定性和定量检测LF的比色适配体传感器。结果 在最优条件下, 20 min内即可完成检测, 在5~75 nmol/L浓度范围内具有良好线性关系, 相关系数(r2)为0.998, 检出限为3.6 nmol/L, 特异性较好, 并且在基质标准曲线中也表现出较好的线性关系(r2=0.987)。结论 该方法操作简单, 分析快速, 安全环保, 灵敏度较高, 有望应用于奶粉中LF的检测。
英文摘要:
      Objective To establish a colorimetric method for the detection of lactoferrin (LF) in milk powder based on aptamer colloidal gold (AuNPs). Methods Using unlabeled LF adaptor as biometric element and AuNPs as signal molecule, a colorimetric adaptor sensor for qualitative and quantitative detection of LF was constructed based on the optical properties of AuNPs. Results Under the optimal conditions, the assay could be completed within 20 min. It had a good linear relationship in concentration range of 5?75 nmol/L, the correlation coefficient (r2) was 0.998, and the limit of detection was 3.6 nmol/L. The specificity was good and it also showed a good linear relationship in the matrix standard curve (r2=0.987). Conclusion The method is simple, rapid, safe, environmentally friendly and sensitive, and is expected to be used for the determination of LF in milk powder.
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