肖之敏,莫益倩,廖雯意,李志龙.超高效液相色谱-串联质谱法测定减肥类保健食品中3种违法添加的缓泻药成分的含量[J].食品安全质量检测学报,2020,11(5):1379-1383
超高效液相色谱-串联质谱法测定减肥类保健食品中3种违法添加的缓泻药成分的含量
Determination for 3 kinds of illegally added laxatives in slimming health foods by ultra performance liquid chromatography-tandem mass spectrometry
投稿时间:2019-12-02  修订日期:2020-03-05
DOI:
中文关键词:  超高效液相色谱-串联质谱法  减肥类  保健食品  缓泻药
英文关键词:ultra performance liquid chromatography-tandem mass spectrometry  slimming  health food  laxative
基金项目:
作者单位
肖之敏 广东省食品工业公共实验室;广东省食品工业研究所有限公司 
莫益倩 广东省食品工业公共实验室;广东省食品工业研究所有限公司 
廖雯意 广东省食品工业研究所有限公司;广东省食品质量监督检验站 
李志龙 广东省食品工业研究所有限公司;广东省食品质量监督检验站 
AuthorInstitution
XIAO Zhi-Min Guangdong Provincial Public Laboratory of Food Industry;Guangdong Food Industry Institute Co., Ltd 
MO Yi-Qian Guangdong Provincial Public Laboratory of Food Industry;Guangdong Food Industry Institute Co., Ltd 
LIAO Wen-Yi Guangdong Food Industry Institute Co., Ltd;Guangdong Food Quality Supervision and Inspection Station 
LI Zhi-Long Guangdong Food Industry Institute Co., Ltd;Guangdong Food Quality Supervision and Inspection Station 
摘要点击次数: 110
全文下载次数: 60
中文摘要:
      目的 建立超高效液相色谱-串联质谱法测定减肥类保健食品中脱乙酰比沙可啶、双醋酚丁和比沙可啶3种违法添加的缓泻药含量的方法。方法 样品用甲醇超声提取后, 十八烷基硅烷键合硅胶色谱柱分离, 乙腈-0.1%甲酸水作为流动相洗脱, 以电喷雾离子源正离子模式电离, 结合多反应监测模式采集多个离子对进行测定。结果 3种缓泻药成分在1.0~100 μg/L范围内线性关系良好, 相关系数均大于0.9991, 平均回收率为82.8%~94.6%, 相对标准偏差为4.52%~6.33%, 方法检出限均为25 μg/kg, 定量限均为80 μg/kg。结论 该方法回收率高, 灵敏度高, 重现性好, 操作简便, 适用于实验室的批量测定。
英文摘要:
      Objective To establish a method for determination of 3 kinds of illegal laxatives of deacetyl bisacridine, bisacetophenol and bisacridine in slimming health foods by ultra performance liquid chromatography- tandem mass spectrometry (UPLC-MS). Methods After ultrasonic extraction with methanol, the samples were separated by octyl silane bonded silica gel chromatographic column, and acetonitrile-0.1% formic acid water was eluted as mobile phase. The samples were ionized by electrospray ion source positive ion mode, and multiple ion pairs were collected in combination with multi-reaction monitoring mode for determination. Results The linear relationship of the 3 cathartic ingredients was good in the range of 1.0~100 μg/L, the correlation coefficient was all more than 0.9991, the average recoveries were 82.8%-94.6%, and the relative standard deviations were 4.52%-6.33%, the limit of detection was 25 μg/kg, the limit of quantitative was 80 μg/kg. Conclusion This method has high recovery rate, high sensitivity, good reproducibility, and simple operation. It is suitable for batch determination in the laboratory.
查看全文  查看/发表评论  下载PDF阅读器