刘铭丽,孔 聪,杨光昕,沈晓盛.河鲀中河鲀毒素检测研究进展[J].食品安全质量检测学报,2020,11(5):1400-1407
河鲀中河鲀毒素检测研究进展
Research progress of tetrodotoxin detection in pufferfish
投稿时间:2020-01-08  修订日期:2020-03-07
DOI:
中文关键词:  河鲀毒素  水产品  食品有害物质检测  生物毒素  免疫电化学检测
英文关键词:tetrodotoxin  aquaticproduct  harmful residue detection of food  biotoxin  electrochemical immunoassay
基金项目:国家自然科学基金项目(31701698)、上海市法医学重点实验室(司法鉴定科学研究院, 开放课题)(KF1910)
作者单位
刘铭丽 中国水产科学研究院东海水产研究所, 农业农村部水产品质量安全风险评估实验室(上海);上海海洋大学食品学院 
孔 聪 中国水产科学研究院东海水产研究所, 农业农村部水产品质量安全风险评估实验室(上海);上海市法医学重点实验室, 司法鉴定科学研究院 
杨光昕 中国水产科学研究院东海水产研究所, 农业农村部水产品质量安全风险评估实验室(上海) 
沈晓盛 中国水产科学研究院东海水产研究所, 农业农村部水产品质量安全风险评估实验室(上海) 
AuthorInstitution
LIU Ming-Li Laboratory of Quality & Safety Risk Assessment for Aquatic Products (Shanghai), Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, East China Sea Fisheries Research Institute;College of Food Science and Technology, Shanghai Ocean University 
KONG Cong Laboratory of Quality & Safety Risk Assessment for Aquatic Products (Shanghai), Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, East China Sea Fisheries Research Institute;Shanghai Key Laboratory of Forensic Medicine, Academy of Forensic Science 
YANG Guang-Xin Laboratory of Quality & Safety Risk Assessment for Aquatic Products (Shanghai), Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, East China Sea Fisheries Research Institute 
SHEN Xiao-Sheng Laboratory of Quality & Safety Risk Assessment for Aquatic Products (Shanghai), Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, East China Sea Fisheries Research Institute 
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中文摘要:
      河鲀毒素(tetrodotoxin, TTX)是一种毒性极强的天然小分子生物碱, 无特效解毒剂, 中毒潜伏期短, 在水产品质量安全领域受到极大关注。本文总结了河鲀毒素的毒性、相关法规、化学性质和反应特点, 并对河鲀毒素检测过程中样品前处理以及检测方法进行了总结, 并详细综述了河鲀毒素常用的生物检测法、液相色谱荧光检测法、气相色谱串联质谱法、液相色谱串联质谱检测法和免疫及电化学检测方法应用情况, 尤其对免疫检测与电化学技术结合的方法进行了分类分析。最后, 本文分析了免疫分析过程中竞争型和非竞争型免疫的区别与优缺点, 并对新型电化学免疫快速检测的方法开发进行了展望, 以期为检测TTX等小分子化合物的新型检测技术的开发提供参考。
英文摘要:
      Tetrodotoxin (TTX) is a highly toxic natural molecule alkaloid with no special antidote and short incubation period. It has attracted great attention in the field of aquatic product safety. In this review, the toxicity, related laws and regulations, chemical structure and properties, and its reaction property of tetrodotoxin were summarized. Furthermore, the development of sample pretreatment method for various detection techniques was introduced. The review also detailed the technology of common bioassay, liquid chromatography with fluorescence detection,gas chromatography-tandem mass spectrometry, liquid chromatography-tandem mass spectrometry,as well as the rapid immunoassay, and the electrochemical method. In particular, the various electrochemistry involved immunoassay were categorized and compared in detail. Finally, the differences, advantages and disadvantages of competitive and non-competitive immunoassay were elaborated and the new electro-immunoassay for TTX was proposed in order to provide a possible strategy for the detection of TTX and other small molecules.
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