何军风.高效液相色谱法检测葡萄酒中柠檬酸[J].食品安全质量检测学报,2018,9(10):2512-2515
高效液相色谱法检测葡萄酒中柠檬酸
Determination of citric acid in wine by high performance liquid chromatography
投稿时间:2018-01-30  修订日期:2018-05-17
DOI:
中文关键词:  高效液相色谱法  葡萄酒  柠檬酸
英文关键词:high performance liquid chromatography  wine  citric acid
基金项目:
作者单位
何军风 平顶山市质量技术监督检验测试中心 
AuthorInstitution
HE Jun-Feng Pingdingshan Quality and Technology Supervision and Inspection Center 
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中文摘要:
      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定葡萄酒中柠檬酸的含量的分析方法。方法 以0.02 mol/L甲醇和磷酸二氢钾(5:95, V:V)为流动相, 流速为0.7 mL/min, 在此色谱条件下, 采用二级管阵列检测器进行检测。结果 在0.05~1.00 g/L范围内, 浓度与峰面积的线性关系良好, 相关系数为0.9999。在加标浓度为5.0、20.0、50.0 g/L时的回收率为93.9%~106.3%, 相对标准偏差(relative standard deviation, RSD)为1.67%~2.99%之间。结论 该方法具有较高的灵敏度和准确性, 且操作简单, 适用于检测葡萄酒样品中的柠檬酸。
英文摘要:
      Objective To establish a method for determination of the content of citric acid in wine by high performance liquid chromatography method (HPLC). Methods The mobile phase was 0.02 mol/L methanol and potassium dihydrogen phosphate (5:95, V:V), and the flow rate was 0.7 mL/min. The sample was detected by the secondary tube array detector. Results In the range of 0.05~1.00 g/L, the linear relationship between the concentration of citric acid and the peak area was good, and the correlation coefficient was 0.9999. At the spiked levels of 5, 20, and 50 g/L, the recovery rates were 93.9%-106.3%, and the relative standard deviation (RSD) was 1.67%-2.99%. Conclusion This method has high sensitivity and accuracy, and simple operation, which is suitable for detecting citric acid in wine samples.
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